Chemical Sciences Molecular Fluorescence Spectroscopy Experiments

Fluorescence Quenching and Stern-Volmer Plot: Estimation of the Quenching Constant from Stern-Volmer Plot

  1. To study how fluorescence intensity is affected by the concentration of quenching agent.
  2. To estimate the quenching constant (KSV) from Stern-Volmer plot: F0/F =1+ KSV[Q]