Chemical Sciences

Introduction

Objective

List of experiments

Target Audience

Acknowledgement

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Molecular Fluorescence Spectroscopy

  1. Introduction to the fluorescence spectroscopy principle

  2. Familiarization with the fluorescence Instrumentation

  3. Familiarization with Excitation and Emission Spectra, Mirror Image Relation and Stokes Shift

  4. Demonstration of Solvent Effects on Fluorescence Spectra of a Fluorophore

  5. Demonstration of pH Effect on Fluorescence Excitation and Emission Spectra of a Fluorophore

  6. Effects of Fluorophore Concentration on Fluorescence Spectra: Inner Filter Effects

  7. Relationship between the Concentration of a Fluorophore and its Fluorescence Intensity: Determination of Unknown Concentration of an Analyte

  8. Determination of Fluorescence Quantum Yield of a Fluorophore

  9. Fluorescence Quenching and Stern-Volmer Plot: Estimation of the Quenching Constant from Stern-Volmer Plot

  10. Effects of Excimer and Exciplex Formations on Fluorescence Emission